magnetic separator untuk 96 well plate takara

  • Phosphopeptide Enrichment MagBeads IMAC based Cube

    Each plate can be rotated into position under a 96-pin magnetic head that drops down inside the 96-well plate to release bind or agitate the magnetic microspheres in solution. In the first robotic run peptides are captured from lysates by carboxylated magnetic beads purified and eluted by digestion at 37°C.

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  • Aksesori Pemipetan Produktivitas Tinggi

    Description Magnetic bead separator plate 96 posts Order No. Material No. Membandingkan Spesifikasi Lengkap 96 Well Plate 2.2 mL Strl LR-P2-96-M-5-S Type Polymeric sealing mat Volume Range N/A Description Sterile polymeric

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  • RAD52 aptamer regulates DNA damage repair and STAT3

    suspension was instilled in the column of the magnetic bead separator (#130‑042‑801 Miltenyi Biotec). The fluid through 96-well plates at 100 µl/well and incubated for 24 h. A 10 µl volume of CCK-8 reagent (#CK04 Dojindo) was added to in a 24-well plate at 1 ml/well and cultured in an incubator for 14 days. Cell number >100

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  • Frontiers Tubeimoside I Antagonizes Yoda1-Evoked Piezo1

     · Measurements were carried out on a 96-well fluorescence plate reader (FlexStation Molecular Devices Sunnyvale CA USA). The change (Δ) in intracellular calcium was calculated based on the ratio of Fura-2 emission (510 nm) intensities excited at 340 and 380 nm and for Fluo-4AM emission (515 nm) excited at 495 nm.

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  • Magnetic-assisted biotinylated single-chain variable

     · A 96-well plate was coated with 100 μL of AMD-BSA (1 8000) at 4 °C for 12 h. The plate was blocked with 5 skim milk then 50 μL AMD standard at different concentrations and 50 μL scFv-BIO was added (50 μL/well) and incubated at 37 °C for 1 h. After washing SA-HRP (1 5000 100 μL/well) was added to each well at 37 °C.

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  • Targeted delivery of neural progenitor cell-derived

     · Then the magnetic beads were collected with a magnetic separator washed and resuspended by PBS. and centrifuged at 10 000 g for 10 min. Fifty microliters of the supernatant was plated in triplicates into a white 96-well plate. Gluc activity was measured by a GloMax luminometer (Promage) with automated injection of 50 μL of 10 ng/mL CTZ

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  • Mapping the Mouse Cell Atlas by Microwell-Seq

     · The workflow of Microwell-seq is shown in Figure 1A. An agarose plate with 10 5 microwells is used to trap 5–10 K individual cells similar to the Cytoseq platform (Fan et al. 2015).After cells (50–100 K) are loaded into the wells the microwell array is inspected under a microscope and rare cell doublets are washed out with a capillary tube.

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  • Takara Bio—Home

    Takara Bio USA Inc. provides kits reagents instruments and services that help researchers explore questions about gene discovery regulation and function. As a member of the Takara Bio Group TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology.

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  • High-throughput full-length single-cell RNA-seq automation

     · Add 24 μL of Agencourt AMPure XP beads to each well of the 96-well plate containing the PCR-amplified cDNA molecules (0.6 1 ratio) and mix well by pipetting. 22 Incubate the mixture at room

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  • NGS clean-up and size selectionTakara Bio

     · Remove the 96-well plate from the NucleoMag®SEP magnetic separator. Add 10–50 μL elution buffer and resuspendthe pellet by pipetting up and down 10 times or by shaking (e.g. at 1100 rpm using an eppendorf Thermomixer®). Incubatethe separation plate at room temperature for 2–5 min.

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  • Effect of a subset of adipose‐derived stem cells isolated

     · The cells were digested with trypsin resuspended counted with a blood cell haemocytometer and added to a 96-well plate at 1000 cells/well in 200 μL medium the cells were treated with 2.5 5 or 10 μmol/L CD146 LMB with DMSO as control and cultured in the CO 2 incubator for 24 hours 20 μL MTT was added and incubated for 2 hours the

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  • Identification of a peptide targeting CD56ScienceDirect

     · Human CD56 protein (5 μg/mL) were added to a 96-well plate for incubation overnight at 4 °C. The plate was incubated with candidate phages for 1 h at 37 °C and 100 μl T7 tail-fiber antibody (1 2500 diluted Novagen Germany) for 1 h at room temperature.

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  • Development Characterization and Evaluation of a

     · After installation of the syringe into a magnetic separator the washing procedure was carried out by importing and removing the magnetic plate from the separator. Dissociation of KGLP-1/HSA was facilitated with 0.1M Gly-HCl buffer (pH 2.5) and the eluate was dialyzed and lyophilized. Finally the IMCMSs were washed with PBS containing

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  • Magnetic Separation Equipment Microcentrifuge Racks for

    Centrifuge Magnetic Separators. 15 mL 50 mL and Combination Racks. Microfuge Tube Separation Racks. 6 12 and 24-tube 1.5 mL Microfuge tube racks and combination 1.5 mL/ 0.2 mL PCR strip. 0.2 mL PCR Strip Separators. PCR strip racks combination 1.5 mL Microcentrifuge/ PCR strip and PCR Strip/ Chiller block. Centrifuge Magnetic Separators.

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  • Characterisation of a subpopulation of CD133 cancer stem

     · CD133 and CD133 − cells were inoculated into a 96-well plate at 500 cells/well in triplicate and cultured in a 37 °C 5 CO 2 incubator. Cell proliferation was measured daily for 7 days. Cell

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  • Efficient Magnet Based Separation of DNA RNA and Protein

    James Maliakal Business. Purifying DNA RNA and protein molecules are at the heart of diagnostics and molecular biology companies. This involves moving or separating the target molecule such as DNA RNA or protein efficiently from a complex mixture. Increasingly magnet based separation is employed for this purpose. Magnets allow movements.

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  • Binding wash and elution plates for NucleoSpin 96 kits

    NucleoSpin® 96 Virus Plates. 4 x 96 Preps. USD 650.00. 96-well silica membrane plates for high-throughput purification of viral RNA and DNA via processing by vacuum or centrifugation. . # 740691.4S consists of a pack of four plates. Notice to purchaser. Our products are to be used for Research Use Only.

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  • Pierce Endogen -

     · Pierce Endogen PRDNO COMMONNAME 10130 MININERT VALVE FITS 25 40 ML SCREW CAP VIALS PKG OF 12 10135 Mininert Valve No. SC

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  • Effect of a subset of adipose‐derived stem cells isolated

     · The cells were digested with trypsin resuspended counted with a blood cell haemocytometer and added to a 96-well plate at 1000 cells/well in 200 μL medium the cells were treated with 2.5 5 or 10 μmol/L CD146 LMB with DMSO as control and cultured in the CO 2 incubator for 24 hours 20 μL MTT was added and incubated for 2 hours the

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  • What Is The Purpose Of Magnetic Separation

    The magnetic separator is one of commonly used preparation equipments in magnetic separation factory. It has dry and wet two types. According to types of magnets it can be divided into permanent magnet type and electromagnet type. It is also can be divided into strong magnet type middle magnet type and weak magnet type according .

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  • SHIMADZU CORPORATION

     · Shimadzu Advanced Healthcare describes the synergistic combination of Shimadzu s core technologies in analytical science and medical diagnostic imaging for healthcare applications including disease prevention diagnosis treatment and prognosis as well as drug discovery. Shimadzu Review. We are offering summaries of the technical publication

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  • Pierce Endogen -

     · Pierce Endogen PRDNO COMMONNAME 10130 MININERT VALVE FITS 25 40 ML SCREW CAP VIALS PKG OF 12 10135 Mininert Valve No. SC

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  • Efficient Magnet Based Separation of DNA RNA and Protein

    James Maliakal Business. Purifying DNA RNA and protein molecules are at the heart of diagnostics and molecular biology companies. This involves moving or separating the target molecule such as DNA RNA or protein efficiently from a complex mixture. Increasingly magnet based separation is employed for this purpose. Magnets allow movements.

    Obrolan Daring
  • High-throughput full-length single-cell RNA-seq automation

     · Add 24 μL of Agencourt AMPure XP beads to each well of the 96-well plate containing the PCR-amplified cDNA molecules (0.6 1 ratio) and mix well by pipetting. 22 Incubate the mixture at room

    Obrolan Daring
  • Targeted delivery of neural progenitor cell-derived

     · Then the magnetic beads were collected with a magnetic separator washed and resuspended by PBS. and centrifuged at 10 000 g for 10 min. Fifty microliters of the supernatant was plated in triplicates into a white 96-well plate. Gluc activity was measured by a GloMax luminometer (Promage) with automated injection of 50 μL of 10 ng/mL CTZ

    Obrolan Daring
  • DynaMag™-2 MagnetThermo Fisher

    The DynaMag-2 magnet is optimized for efficient magnetic separation of all types of Dynabeads (range 14.5 m in diameter) in small (2 mL) sample volumes. The DynaMag-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart er

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  • Phosphopeptide Enrichment MagBeads IMAC based Cube

    Each plate can be rotated into position under a 96-pin magnetic head that drops down inside the 96-well plate to release bind or agitate the magnetic microspheres in solution. In the first robotic run peptides are captured from lysates by carboxylated magnetic beads purified and eluted by digestion at 37°C.

    Obrolan Daring